pSB1C3_I0500_LuxI vector (V003461)

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LOCUS       V003461                 3891 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003461
VERSION     V003461
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge,
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 3891)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..879)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        906..1190
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     regulatory      1219..1230
                     /label="BBa_B0034"
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             1219..1230
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1237..1815
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     misc_feature    1822..1842
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      1843..1900
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2866..2960)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2984..3640)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3641..3744)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3824..3867)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    3870..3891
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"