pSAT5-EGFP-C1 vector (V003494)

LOCUS       40924_38553        4589 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT5-EGFP-C1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        505..1176
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1186..1316
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     CDS             1319..2035
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    2036..2101
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2114..2324
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2368..2384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2392..2408)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2416..2446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2461..2482)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2770..3358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3532..4389)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4390..4494)
                     /label=AmpR promoter