pSal43A vector (V003514)

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LOCUS       V003514                 5873 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003514
VERSION     V003514
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5873)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5873)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    651..1735
                     /label="5' flanking region of A. nidulans argB"
                     /note="5' flanking region of A. nidulans argB"
     misc_feature    1736..1788
                     /label="5' UTR of A. nidulans argB"
                     /note="5' UTR of A. nidulans argB"
     CDS             1789..2865
                     /gene="argB"
                     /label="Ornithine carbamoyltransferase, mitochondrial"
                     /note="Ornithine carbamoyltransferase, mitochondrial from
                     Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS
                     112.46 / NRRL 194 / M139). Accession#: P11803"
     regulatory      2869..3255
                     /label="3' UTR of A. nidulans argB, incl. polyA"
                     /note="3' UTR of A. nidulans argB, incl. polyA"
                     /regulatory_class="terminator"
     rep_origin      complement(4046..4634)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4808..5665)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5666..5770)
                     /label="AmpR promoter"