pSac-Cm vector (V003516)

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LOCUS       V003516                 5045 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003516
VERSION     V003516
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
   PUBMED   15109830
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 5045)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5045)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..700
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus
                     subtilis"
     CDS             complement(1115..1762)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    2257..2733
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus
                     subtilis"
     promoter        3139..3243
                     /label="AmpR promoter"
     CDS             3244..4101
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4275..4863
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"