pSA91 vector (V003520)

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LOCUS       V003520                 5071 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003520
VERSION     V003520
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Auslaender S, Auslaender D, Mueller M, Wieland M, Fussenegger M.
  TITLE     Programmable single-cell mammalian biocomputers
  JOURNAL   Nature 487 (7405), 123-127 (2012)
   PUBMED   22722847
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Auslaender S, Auslaender D, Mueller M, Wieland M, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2012) Department of Biosystems Science and
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058,
            Switzerland
REFERENCE   3  (bases 1 to 5071)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5071)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature";
            date: "2012"; volume: "487"; issue: "7405"; pages: "123-127"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-FEB-2012) Department of Biosystems Science and Engineering,
            D-BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        188..226
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        348..366
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             390..746
                     /gene="rpl7ae"
                     /label="Large ribosomal subunit protein eL8"
                     /note="Large ribosomal subunit protein eL8 from
                     Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM
                     9628 / NBRC 100126 / VC-16). Accession#: O29494"
     polyA_signal    793..1017
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1063..1491
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1505..1834
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             1901..2692
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    2869..3002
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3039..3055)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3063..3079)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3087..3117)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3132..3153)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3441..4026)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4200..5057)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(join(5058..5071,1..91))
                     /label="AmpR promoter"