pRvdB1 vector (V003538)

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LOCUS       40924_38318        7039 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pRvdB1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Van Den Bekerom RJM., Hogg B, McCabe MS.
  TITLE     Transformation vector with loxp-sites and aadA for targeting the 
            trnA and trnI region of the tobacco chloroplast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Van Den Bekerom RJM., Hogg B, McCabe MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2005) Biology Department, National University of 
            Ireland, Maynooth, Maynooth, County Kildare, Ireland
REFERENCE   3  (bases 1 to 7039)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2005) Biology Department, National University of Ireland, 
            Maynooth, Maynooth, County Kildare, Ireland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            46..827
                     /pseudo
                     /gene="trnA"
                     /label=trnA
     promoter        complement(847..865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(872..888)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1029..1457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             1801..2592
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2613..3470
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3644..4232
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4520..4541
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4556..4586
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4594..4610
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4618..4634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4652..4670
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     gene            4750..5528
                     /pseudo
                     /gene="trnI"
                     /label=trnI
     protein_bind    5545..5578
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5626..5759
                     /label=Prrn promoter in tobacco plastome
                     /note="Prrn promoter in tobacco plastome"
                     /regulatory_class="promoter"
     CDS             5760..6548
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     3'UTR           6564..6956
                     /label=PsbA-3'UTR
                     /note="PsbA-3'UTR"
     misc_feature    6998..7033
                     /label=loxP site
                     /note="loxP site"
     protein_bind    complement(6999..7032)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."