pRS52 vector (V003552)

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LOCUS       40924_38158        4295 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRS52, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ.
  TITLE     Phosphatase activity tunes two-component system sensor detection 
            threshold
  JOURNAL   Nat Commun 9 (1), 1433 (2018)
  PUBMED    29650958
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Landry BP.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2017) Bioengineering, Rice University, 6100 Main 
            St, Houston, TX 77005, USA
REFERENCE   3  (bases 1 to 4295)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4295)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2018"; volume: "9"; issue: "1"; pages: "1433"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-2017) Bioengineering, Rice University, 6100 Main St, 
            Houston, TX 77005, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..58
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(364..1311)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     repeat_region   1313..1359
     regulatory      complement(1342..1347)
                     /regulatory_class="minus_10_signal"
     rep_origin      complement(1359..1581)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     misc_feature    complement(1693..2060)
                     /label=par region
                     /note="par region"
     repeat_region   1959..1976
     repeat_region   1982..2005
     repeat_region   2016..2032
     misc_feature    complement(2035..2039)
                     /label=Tn3 integration site
                     /note="Tn3 integration site"
     repeat_region   2045..2058
     misc_feature    complement(2070..2071)
                     /label=GC>CA
                     /note="GC>CA"
     terminator      complement(2073..2167)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2200..3057)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3058..3162)
                     /label=AmpR promoter
     misc_feature    3208..3245
                     /label=BW25113 LacZ Upstream
                     /note="BW25113 LacZ Upstream"
     terminator      complement(3249..3292)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      3295..3440
                     /label=PompF
                     /note="PompF"
                     /regulatory_class="promoter"
     protein_bind    3306..3325
                     /label=OmpR-F1 binding site
                     /bound_moiety="OmpR-F1"
     regulatory      3308..3313
                     /regulatory_class="minus_10_signal"
     protein_bind    3326..3345
                     /label=OmpR-F2 binding site
                     /bound_moiety="OmpR-F2"
     regulatory      3334..3339
                     /regulatory_class="minus_35_signal"
     protein_bind    3346..3365
                     /label=OmpR-F3 binding site
                     /bound_moiety="OmpR-F3"
     regulatory      3354..3359
                     /regulatory_class="minus_35_signal"
     regulatory      3357..3362
                     /regulatory_class="minus_10_signal"
     regulatory      3371..3376
                     /regulatory_class="minus_35_signal"
     regulatory      3382..3387
                     /regulatory_class="minus_10_signal"
     regulatory      3394..3399
                     /regulatory_class="minus_10_signal"
     regulatory      3406
                     /label=+1 nucleotide
                     /note="+1 nucleotide"
                     /regulatory_class="promoter"
     CDS             3441..4154
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     terminator      4175..4246
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    4247..4254
                     /label=BB Scar
                     /note="BB Scar"
     terminator      4262..4289
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"