pTn5.7luxTc6 vector (V002594)

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LOCUS       40924_43663       11969 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter capture vector pTn5.7luxTc6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux : A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 11969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_feature    complement(1276..1294)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     CDS             complement(1449..2876)
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     regulatory      complement(3019..3048)
                     /label=PS12
                     /note="PS12"
                     /regulatory_class="promoter"
     misc_feature    3055..3073
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     protein_bind    3083..3107
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3137..4576
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             4591..5511
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             5563..6642
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6660..7640
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             7822..8931
                     /label=LuxE
                     /note="LuxE"
     protein_bind    complement(8969..8993)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     9049..9096
                     /label=FRT
                     /note="FRT"
     protein_bind    complement(9049..9096)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(9237..10424)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(10472..10500)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     protein_bind    10747..10768
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10783..10813
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10821..10837
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10845..10861
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10879..10897
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    complement(10965..11012)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(11058..11144)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(11247..11341)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(11369..11385)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11390..11588
                     /label=Tn7R
                     /note="Tn7R"