pTM117 vector (V002608)

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LOCUS       40924_43598       11278 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTM117, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Carpenter BM, McDaniel TK, Whitmire JM, Gancz H, Guidotti S, Censini
            S, Merrell DS.
  TITLE     Expanding the Helicobacter pylori Genetic Toolbox: Modification of 
            an Endogenous Plasmid for Use as a Transcriptional Reporter and 
            Complementation Vector
  JOURNAL   Appl. Environ. Microbiol. 73 (23), 7506-7514 (2007)
  PUBMED    17921278
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Carpenter BM, McDaniel TK, Whitmire JM, Gancz H, Guidotti S, Censini
            S, Covacci A, Merrell DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2007) Microbiology and Immunology, Uniformed 
            Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
REFERENCE   3  (bases 1 to 11278)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2007"; volume: "73"; issue: "23"; 
            pages: "7506-7514"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-APR-2007) Microbiology and Immunology, Uniformed Services 
            University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..738)
                     /codon_start=1
                     /gene="r2"
                     /product="putative cAMP-induced filamentation protein"
                     /label=r2
                     /note="R2"
                     /protein_id="ABQ18301.1"
                     /translation="MKRQLHLSKLIEKLNHERKNAIKNGIYHLIQIKFSYNSNRIEGSG
                     LTYEQTAHIFDKSVLITEKNANIKLDDIFETINHFECVNYLLESYQESLSLEYFKTLHK
                     ILKKNCSDEVIGGFKKRPNFVGNSATTRPQLVESELTNLVKNYQRNLEVSLENIIDFHV
                     AFEKIHPFSDGNGRVGRLVMFKECLKNNIMPFIIENEHKAFYYRGVKEYHNTKGYLKDT
                     ILQSQDNFNEMVSYFFSK"
     gene            complement(16..738)
                     /gene="r2"
                     /label=r2
     CDS             complement(735..2339)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA replicase"
                     /label=repA
                     /note="RepA"
                     /protein_id="ABQ18302.1"
                     /translation="MEFDQLESQRSDLQKVLKELDTLPKTPQIELQKQEIQDRINKITD
                     TIIKELLSKHEIKKEELEPTLKEKPTPTKVPQTTPTPCKDLVVSTPKDKTYTTYHNNTN
                     KVNLGKLSEREANLLFAIFQMLKDQGNTLIRFEPQDLKRMLNIDISNERLSEVVIKLWD
                     SIKTADFWKISETETSIIQENYMLFSRCKIELNKPSKDLKYLEIQLNDNYQYLLNNLGM
                     GQYTSFNLLEFQRVRGKYAKTLYRLLKQYKSTGILSVEWGQFRELLDIPKDYDMRSIDN
                     FVLKIALKELHKIYPFEHLSYKKERRNSHDRRKVTHIDFYFEQLPKGETKKQKQADKQR
                     AKRDIKLIAWDIHKEIAKRNAKDTMEARFLELKTLIGYQFRHNDSGIVLQIDNTAFEKN
                     QMFLHVSYPKSKHNPQKFLVSNKTFALELLYINGYSLKKDNLLEEIDPPKIHPITNEPI
                     KEFAQYIGKTINITNFNVDQCPKGINNYLKITRIVKLNDNRICISVQDVDKPEKLLKPF
                     IAKDEKHLNNWFKKHYR"
     gene            complement(735..2339)
                     /gene="repA"
                     /label=repA
     CDS             1104..1490
                     /codon_start=1
                     /gene="f3"
                     /product="hypothetical protein"
                     /label=f3
                     /note="F3"
                     /protein_id="ABQ18303.1"
                     /translation="MLAFRVRNMQKHLILFKSGVVNLQNDPTVIVPELIADQSFQFKKP
                     SFHSVFGVSFSDFFVNIPCYELDVPFSALLVGLFLFLGFAFGQLLKVEINVRYLAPVVA
                     VSAFFFVTQVFKRVDFMEFFESDF"
     gene            1104..1490
                     /gene="f3"
                     /label=f3
     rep_origin      2666..2766
                     /label=from pHP666
                     /note="from pHP666"
     CDS             3420..4832
                     /codon_start=1
                     /gene="f4"
                     /product="putative replicase"
                     /label=f4
                     /note="F4"
                     /protein_id="ABQ18304.1"
                     /translation="MLKDYWECFNFLTYNDYEEWSNGEDFYSFIFPNCESKGEINKDFS
                     KPNAVFLYKDLKTTLNDTDKPALKRRIMLKDTWGDDYIDFVLENDLTLCSGLSYRGRHN
                     DLAHAQQMNALIFDLDGVGLKEITAFFEVIKYCEKNEPNKYFWPIPRPTFVVLSGVGLH
                     VYYVFDKPIDLFPNIKLQLKAYKYALTFNIWRYKETSKEKETQYQSINQSFRMVGSINE
                     KHGNKIIAFKTGDRVSLEYMNQYVKEDKRVDILKRFRPSKHTLAEAKLKFPEWYEKVII
                     QGQTSERKKWRIKRDVYEWWKKRCKEPKGGHRYFFMMCMAIYAYKCDVSKQELEKDMLE
                     VFEKLKDIPHTNPLTKRDVYSALESYDKGLACFKIKDIEILTALRIDRNRRNYRQQYLH
                     LKGIRALQQAINPTWRQGNGRPKNSGIKQSLIQEWRIKNPQGKKIDCHKELGLSRPTID
                     KWWNSKQLAMTT"
     gene            3420..4832
                     /gene="f4"
                     /label=f4
     CDS             complement(4806..5072)
                     /codon_start=1
                     /gene="r3"
                     /product="hypothetical protein"
                     /label=r3
                     /note="R3"
                     /protein_id="ABQ18305.1"
                     /translation="MLDIEYHNIFRKDYKKYLKNGFDSKLLDEVVLELRQQKPLAQKHK
                     DHILKGEWYPCRECHIRPDVLLVYIVKNNTLWLLRLGSHSELF"
     gene            complement(4806..5072)
                     /gene="r3"
                     /label=r3
     CDS             complement(5059..5337)
                     /codon_start=1
                     /gene="r4"
                     /product="hypothetical protein"
                     /label=r4
                     /note="R4"
                     /protein_id="ABQ18306.1"
                     /translation="MLKDVEVGVKFYKELGKLEKQLAKYQSKVLEIKKQMREIKKQYSQ
                     AKKDEKKAKNLETLQAIKDVDSGANFTKIDSLDELTNELKREIHAGY"
     gene            complement(5059..5337)
                     /gene="r4"
                     /label=r4
     CDS             5539..5883
                     /codon_start=1
                     /gene="mobC"
                     /product="MobC mobilization protein"
                     /label=mobC
                     /protein_id="ABQ18307.1"
                     /translation="MPKLSDNKNITKRLRLNQTEWQIIQTQMQEKNLNFSQLVLNSLLT
                     QNSQTPTRSKKQKAIANKELVIELAKWGNNLNQIAKCLNTNKGAWDRLGLEQLIEISYQ
                     LEQIRSKYVG"
     gene            5539..5883
                     /gene="mobC"
                     /label=mobC
     CDS             5873..7888
                     /codon_start=1
                     /gene="mobA"
                     /product="MobA mobilization protein"
                     /label=mobA
                     /note="MobA"
                     /protein_id="ABQ18308.1"
                     /translation="MLVKFWGINQGGGDGDGSVNYLLNERVKQGTAKVLKGDANLTKSL
                     LLSLTQKHKACVGCLSFEESNIDESLKYELMESFENALLTQEMQGRYNILWVEHTDKGR
                     LELNFVIPKIDLEKQKAFNPYYHKVDLKRIDTWKDCINLKHNFTNPKDLEKQQISNYHN
                     TKTPQGKAIFKNYKELDNYLYNAVTQGLLNSRAEIIELLQASNYEITRQGKDYISVKLP
                     NQQKAKRLKGIIYDEQFTEQRLIEQELREYSQNKDTSRERDTREISTAPNTTPRATQSL
                     IQELRDLEQQLHRHIEYKQRYYKKLHQSTSKDPREPKEQLPRVRDSQQRTQQNHANETT
                     RARETNPQEPSQEILTNAPNFHHRSSHHGGNISGGILSHHSTKQGQGIRAFTERSLSVK
                     TRNRPTKQRQSTENLQPNADQEIRGKNAGRILKQATERFNQRSRELTERERDLAQRKEQ
                     RNREFRERQKRAINQARESYSRISNAKQRLLEIRGRNLELERELRNAIPNAQHIISQIS
                     SFRESRESRIKSINANLRTERNHAQKRFTEHVRADFTTRITERSNQRARDFTSSIQRIE
                     NTIRDFRNNAQARVRTILHNLYARTRDAINTIKDIYRNAKERIRDFFQRADERLSKRME
                     QEARELHTKTTQLDRKIREHNQEIDDERSYSCGYGR"
     gene            5873..7888
                     /gene="mobA"
                     /label=mobA
     CDS             7949..8083
                     /codon_start=1
                     /gene="f2"
                     /product="hypothetical protein"
                     /label=f2
                     /note="F2"
                     /protein_id="ABQ18309.1"
                     /translation="MKEYKIFISHSWKYDNHYANLQDALKDLAEEIQPKVYEYGFLRE"
     gene            7949..8083
                     /gene="f2"
                     /label=f2
     CDS             complement(8131..8922)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9086..9110
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             9131..9844
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             10022..10210
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    10315..10455
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10641..11229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"