pTL61T vector (V002612)

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LOCUS       cloning_vector;        8565 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTL61T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    cloning vector; synthetic sequence
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Linn T, St Pierre R.
  TITLE     Improved vector system for constructing transcriptional fusions that
            ensures independent translation of lacZ
  JOURNAL   J. Bacteriol. 172 (2), 1077-1084 (1990)
  PUBMED    2137119
REFERENCE   2  (bases 1 to 8565)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8565)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "1990"; volume: "172"; issue: "2"; pages: 
            "1077-1084"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Draft entry and computer-readable sequence kindly submitted by 
            T.G.Linn, 15-NOV-1989.
            Authors indicate an uncharacterized mutation between bp 3274-3379, 
            which alters a EcoRI site normally present in the E.coli lacZ gene.
FEATURES             Location/Qualifiers
     source          1..8565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..92
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    116..192
                     /label=RNaseIII processing site
                     /note="RNaseIII processing site"
     misc_recomb     238..239
     CDS             256..3327
                     /label=lacZ
                     /note="beta-galactosidase"
     misc_recomb     3392..3393
     misc_recomb     3890..3891
     terminator      4091..4177
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4269..4296
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      4589..4675
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4767..4794
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_recomb     4886..4887
     CDS             5378..5566
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5671..5811
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5997..6585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6759..7616)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7617..7721)
                     /label=AmpR promoter
     variation       7824..7826
                     /note="a in pBR322; att in pTL61T"
     misc_recomb     7826..7827
     terminator      7846..7932
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8027..8113
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      8208..8294
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(8392..8435)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"