pTL5 vector (V002613)

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LOCUS       40924_43573        5793 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cosmid vector pTL5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Slightom JL, Siemieniak DR, Koop BF, Hood L.
  TITLE     The complete nucleotide sequence of cosmid vector pTL5: location and
            origin of its genetic components
  JOURNAL   Gene 147 (1), 77-79 (1994)
  PUBMED    8088551
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Sutcliffe JG.
  TITLE     Complete nucleotide sequence of the Escherichia coli plasmid pBR322
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 43 (PT 1), 77-90 (1979)
  PUBMED    383387
REFERENCE   3  (bases 1 to 5793)
  AUTHORS   Daniels DL, Schroeder JL, Szybalski W, Sanger F, Blattner FR.
  TITLE     Appendix I: A Molecular Map of Coliphage Lambda
  JOURNAL   (in) Hendrix,R.W. (Ed.); LAMBDA II: 519-676; Cold Spring Harbor 
            Laboratory, Cold Spring Harbor, NY (1983)
REFERENCE   4  (bases 1 to 5793)
  AUTHORS   Lund T, Grosveld FG, Flavell RA.
  TITLE     Isolation of transforming DNA by cosmid rescue
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79 (2), 520-524 (1982)
  PUBMED    6210908
REFERENCE   5  (bases 1 to 5793)
  AUTHORS   Slightom JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-1994) Jerry L. Slightom, Molecular Biology, The 
            Upjohn Company, 301 Henrietta Street, Kalamazoo, MI 49007, USA
REFERENCE   6  (bases 1 to 5793)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 5793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1994"; volume: "147"; issue: "1"; pages: "77-79"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cold Spring
            Harb. Symp. Quant. Biol. 43 (PT 1), 77-90 (1979)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "(in) 
            Hendrix,R.W. (Ed.)"; volume: " LAMBDA II"; pages: " 519-676; Cold 
            Spring Harbor Laboratory, Cold Spring Harbor, NY (1983"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1982"; volume: "79"; issue: "2"; pages: 
            "520-524"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-1994) Jerry L. Slightom, Molecular Biology, The Upjohn 
            Company, 301 Henrietta Street, Kalamazoo, MI 49007, USA"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             86..1273
                     /label=TcR
                     /note="tetracycline efflux protein"
     terminator      1537..1631
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    2114..3448
                     /note="Sequence region obtained from bacteriophage Charon
                     4A, but originally from bacteriophage phi 80. phi 80 right 
                     arm region"
     misc_feature    3437..3448
                     /label=bacteriophage Charon 4A cohesive ends
                     /note="bacteriophage Charon 4A cohesive ends"
     misc_feature    3449..3854
                     /note="DNA region from Charon 4A, originally from
                     bacteriophage lambda, left arm region"
     misc_feature    3573
                     /note="left arm of bacteriophage lambda (GenBank Accession 
                     Number J02459) differs after this position as it contains 
                     one additional G nucleotide not found in pTL5"
     CDS             4102..4290
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4395..4535
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4721..5308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"