pTKRED vector (V002615)

Download GeneBank File(.gb)

LOCUS       V002615                10726 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002615
VERSION     V002615
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Kuhlman TE, Cox EC.
  TITLE     Site-specific chromosomal integration of large synthetic constructs
  JOURNAL   Nucleic Acids Res. 38 (6), E92 (2010)
   PUBMED   20047970
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   Kuhlman TE, Cox EC.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2009) Molecular Biology, Princeton University,
            Washington Rd., Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 10726)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10726)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2010"; volume: "38"; issue: "6"; pages: "E92"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-DEC-2009) Molecular Biology, Princeton University, Washington
            Rd., Princeton, NJ 08544, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(147..1022)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        1049..1333
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     RBS             1360..1368
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1383..2075
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     CDS             2663..3451
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             complement(3781..4458)
                     /label="Exo"
                     /note="5' to 3' double-stranded DNA exonuclease in the
                     lambda Red system"
     CDS             complement(4458..5240)
                     /label="Beta"
                     /note="single-stranded DNA binding recombinase in the
                     lambda Red system"
     CDS             complement(5249..5662)
                     /label="Gam"
                     /note="inhibitor of the host RecBCD nuclease in the lambda
                     Red system"
     regulatory      complement(5687..5714)
                     /label="Plac"
                     /note="Plac"
                     /regulatory_class="promoter"
     protein_bind    5696..5712
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5720..5750)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             complement(5879..6937)
                     /label="RecA"
                     /note="DNA repair protein from E. coli"
     CDS             complement(7429..8508)
                     /label="lacI"
                     /note="lac repressor"
     regulatory      complement(8542..8574)
                     /label="PlacI"
                     /note="PlacI"
                     /regulatory_class="promoter"
     CDS             complement(8697..9644)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(9692..9914)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"