Basic Vector Information
- Vector Name:
- pTK251
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 12543 bp
- Type:
- Plant transient expression vector
- Replication origin:
- oriV
- Host:
- Plants
- Source/Author:
- Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH, Rozwadowski K.
- Promoter:
- CaMV 35S (enhanced)
pTK251 vector Vector Map
pTK251 vector Sequence
LOCUS V002622 12543 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V002622
VERSION V002622
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 12543)
AUTHORS Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
Rozwadowski K.
TITLE TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
expression vectors for functional analysis of proteins
JOURNAL Sci Rep 2, 874 (2012)
PUBMED 23166857
REFERENCE 2 (bases 1 to 12543)
AUTHORS Rozwadowski K, Kagale S.
TITLE Direct Submission
JOURNAL Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
0X2, Canada
REFERENCE 3 (bases 1 to 12543)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12543)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
874 (2012)"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12543
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 5..29
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
primer_bind 110..126
/label="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
promoter 230..899
/label="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 911..966
/label="TMV Omega"
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 968..4318
/codon_start=1
/gene="Rep"
/product="replicase"
/label="Rep"
/protein_id="AFX83519.1"
/translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
MYKVDAGTQ"
gene 968..4318
/gene="Rep"
/label="Rep"
CDS 4394..5812
/codon_start=1
/gene="RNA pol"
/product="RNA polymerase"
/label="RNA pol"
/protein_id="AFX83520.1"
/translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
KVLFRSLFIDGSSC"
gene 4394..5812
/gene="RNA pol"
/label="RNA pol"
CDS 5796..6599
/gene="MP"
/label="Movement protein"
/note="Movement protein from Tobacco mosaic virus (strain
vulgare). Accession#: P03583"
CDS 6683..6706
/label="Strep-Tag II"
/note="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
CDS 6743..6766
/label="Strep-Tag II"
/note="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
CDS 6800..6817
/label="6xHis"
/note="6xHis affinity tag"
CDS 6821..6853
/label="T7 tag (gene 10 leader)"
/note="leader peptide from bacteriophage T7 gene 10"
CDS 6857..6880
/label="Xpress(TM) tag"
/note="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
protein_bind 6890..7014
/label="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 7039..7069
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
CDS 7123..7779
/label="CmR"
/note="chloramphenicol acetyltransferase"
CDS 8124..8426
/label="ccdB"
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(8470..8594)
/label="attR2"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 8839..8883
/note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
virus satellite RNA and Hammerhead ribozyme G12a"
regulatory 8926..9136
/label="CaMV 35S"
/note="CaMV 35S"
/regulatory_class="terminator"
misc_feature 9206..9230
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS complement(9385..10530)
/label="trfA"
/note="trans-acting replication protein that binds to and
activates oriV"
CDS complement(10832..11623)
/label="KanR"
/note="aminoglycoside phosphotransferase"
rep_origin complement(11835..12470)
/direction=LEFT
/label="oriV"
/note="incP origin of replication"
This page is informational only.