pTJ1 vector (V002626)

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LOCUS       40924_43468        5667 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTJ1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Damron FH, McKenney ES, Schweizer HP, Goldberg JB.
  TITLE     Construction of a Broad-Host-Range Tn7-Based Vector for Single-Copy 
            PBAD-Controlled Gene Expression in Gram-Negative Bacteria
  JOURNAL   Appl. Environ. Microbiol. 79 (2), 718-721 (2013)
  PUBMED    23124231
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Damron FH, McKenney ES, Schweizer H, Goldberg JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2012) Microbiology, Immunology, and Cancer 
            Biology, University of Virginia, PO Box 800734, Charlottesville, VA 
            22908, USA
REFERENCE   3  (bases 1 to 5667)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "2"; pages:
            "718-721"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-2012) Microbiology, Immunology, and Cancer Biology, 
            University of Virginia, PO Box 800734, Charlottesville, VA 22908, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..885)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        912..1196
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             1223..1245
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1257..1259
                     /label=start codon
                     /note="start codon"
     misc_feature    1271..1327
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     mobile_element  complement(1368..1533)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(1692..1800)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(2032..2620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2794..3651)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3652..3756)
                     /label=AmpR promoter
     mobile_element  4234..4431
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
     primer_bind     4436..4452
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4480..4574
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      4677..4763
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    4814..4861
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(4926..5159)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(5484..5512)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   5564..5611
                     /rpt_family="FRT"
     protein_bind    5564..5611
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."