pTIT-N vector (V002628)

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LOCUS       V002628                 4535 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002628
VERSION     V002628
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Finke S, Conzelmann KK.
  TITLE     Virus promoters determine interference by defective RNAs: selective
            amplification of mini-RNA vectors and rescue from cDNA by a 3'
            copy-back ambisense rabies virus
  JOURNAL   J. Virol. 73 (5), 3818-3825 (1999)
   PUBMED   10196276
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS.
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic
            tracing and high-level gene expression in neurons
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4535)
  AUTHORS   Wickersham IR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes
            Medical Institute and Massachusetts Institute of Technology, 43
            Vassar St., Cambridge, MA 02139, USA
REFERENCE   4  (bases 1 to 4535)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "1999"; volume: "73"; issue: "5"; pages: "3818-3825"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes Medical
            Institute and Massachusetts Institute of Technology, 43 Vassar St.,
            Cambridge, MA 02139, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..164
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        171..189
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    240..701
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             706..2055
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Rabies virus (strain SAD B19).
                     Accession#: P16285"
     terminator      2221..2268
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     primer_bind     complement(2291..2307)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2344..2362)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2383..2399)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2407..2423)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2431..2461)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2476..2497)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2785..3373)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3547..4404)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4405..4509)
                     /label="AmpR promoter"