pTip-LCH2.2 vector (V002639)

LOCUS       V002639                 8361 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002639
VERSION     V002639
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     A novel system for expressing recombinant proteins over a wide
            temperature range from 4 to 35 degrees C
  JOURNAL   Biotechnol. Bioeng. 86 (2), 136-148 (2004)
   PUBMED   15052633
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2002) Nobutaka Nakashima, National Institute of
            Advanced Industrial Science and Technology; Tsukisamu-Higashi
            2-17-2-1, Sapporo, Toyohira-ku 062-8517, Japan
            (E-mail:n-nakashima@aist.go.jp, Tel:81-11-857-8937)
REFERENCE   3  (bases 1 to 8361)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8361)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng."; date: "2004"; volume: "86"; issue: "2"; pages: "136-148"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2002) Nobutaka Nakashima, National Institute of Advanced
            Industrial Science and Technology"; volume: " Tsukisamu-Higashi
            2-17-2-1, Sapporo, Toyohira-ku 062-8517, Japan
            (E-mail:n-nakashima@aist.go.jp, Tel"; pages: "81-11-857-8937"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             170..976
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             1541..2716
                     /codon_start=1
                     /gene="Chlr"
                     /product="chloramphenicol resistance protein"
                     /label="Chlr"
                     /protein_id="BAD11299.1"
                     /translation="VPFAIYVLGLAVFAQGTSEFMLSGLIPDMARDLGVSVPAAGLLTS
                     AFAVGMIIGAPLMAIASMRWPRRRALLTFLITFMLVHVIGALTSSFEVLLVTRIVGALA
                     NAGFLAVALGAAMAMVPADMKGRATSVLLGGVTIACVAGVPGGAFLGEMWGWRAAFWAV
                     VVISAPAVVAIMFATPAEPLAESTPNAKRELSSLRSRKLQLMLVLGALINGATFCSFTY
                     MAPTLTDISGFDSRWIPLLLGLFGLGSFIGVSVGGRLADTRPFQLLAVGSAALLTGWIV
                     FALTASHPAVTLVMLFVQGALSFAVGSTLISQVLYAADAAPTLGGSFATAAFNVGAALG
                     PALGGLAIGMGLSYRAPLWTSAALVTLAIVIGAATLSLWRRPASVHESVPA"
     gene            1541..2716
                     /gene="Chlr"
                     /label="Chlr"
     regulatory      2905..3080
                     /gene="ThcA"
                     /regulatory_class="promoter"
     gene            2905..3080
                     /gene="ThcA"
                     /label="ThcA"
     CDS             3081..3839
                     /gene="tipA"
                     /label="HTH-type transcriptional activator TipA"
                     /note="HTH-type transcriptional activator TipA from
                     Streptomyces lividans. Accession#: P0A4T9"
     promoter        4020..4124
                     /label="AmpR promoter"
     CDS             4125..4982
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5156..5744
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      complement(5995..6175)
                     /gene="ThcA"
                     /regulatory_class="terminator"
     gene            complement(5995..6175)
                     /gene="ThcA"
                     /label="ThcA"
     CDS             complement(6189..6206)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     RBS             complement(6262..6284)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      complement(6298..6421)
                     /gene="TipA"
                     /regulatory_class="promoter"
     CDS             6960..7847
                     /codon_start=1
                     /gene="RepA"
                     /label="RepA"
                     /protein_id="BAD11302.1"
                     /translation="MKSATGGVLSDRQWADAVLLPHRPFATNNLQRGQYRMSRDDALAM
                     RYVEHSPHALLGSIVIDCDHVDAAMRAFEQPSDHPAPNWVAQSPSGRAHIGWWLGPNHV
                     CRTDSARLTPLRYAHRIETGLKISVGGDFAYGGQLTKNPIHPDWETIYGPATPYTLRQL
                     ATIHTPRQMPRRPDRAVGLGRNVTMFDATRRWAYPQWWQHRNGTGRDWDHLVLQHCHAV
                     NTEFTTPLPFTEVRATAQSISKWIWRNFTEEQYRARQAHLGQKGGKATTLAKQEAVRNN
                     ARKYDEHTMREAII"
     gene            6960..7847
                     /gene="RepA"
                     /label="RepA"
     CDS             7847..8131
                     /codon_start=1
                     /gene="RepB"
                     /label="RepB"
                     /protein_id="BAD11303.1"
                     /translation="MGGAKNPVRRKMTAAAAAEKFGASTRTIQRLFAEPRDDYLGRAKA
                     RRDKAVELRKQGLKYREIAEAMELSTGIVGRLLHDARRHGEISAEDLSA"
     gene            7847..8131
                     /gene="RepB"
                     /label="RepB"