pTFH22.4 vector (V002736)

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LOCUS       40924_43139        6567 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector pTFH22.4 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Sakakibara K, Reisewitz P, Aoyama T, Friedrich T, Ando S, Sato Y, 
            Nishiyama T, Tamada Y, Hiwatashi Y, Kurata T, Ishikawa M, Deguchi H,
            Rensing SA, Werr W, Murata T, Hasebe M, Laux T.
  TITLE     WOX13-like genes are required for the initiation of apical stem 
            cells from differentiated leaf cells in the moss Physcomitrella 
            patens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Sakakibara K, Fujita T, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2012) Contact:Keiko Sakakibara Graduate School of 
            Science, Hiroshima University, Department of Biological Science; 
            1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan
REFERENCE   3  (bases 1 to 6567)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-OCT-2012) Contact:Keiko Sakakibara Graduate School of Science, 
            Hiroshima University, Department of Biological Science; 1-3-1 
            Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8526, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..616
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    904..925
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        940..970
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    978..994
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1002..1018
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1055..1893
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             1898..1915
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    2450..2976
                     /label=TrbcS
                     /note="TrbcS"
     misc_feature    2461..2496
                     /note="SfiI Multi cloning site (SfiI, AscI, SmaI, ApaI,
                     SfiI)"
     promoter        3055..3402
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3423..4214
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4287..4462
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        5143..5488
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5536..6252
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      6263..6515
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"