pTEXL-Hyg vector (V002743)

LOCUS       40924_43104        6174 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTEXL-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Bouvier LA, Camara Mde L, Canepa GE, Miranda MR, Pereira CA.
  TITLE     Plasmid Vectors and Molecular Building Blocks for the Development of
            Genetic Manipulation Tools for Trypanosoma cruzi
  JOURNAL   PLoS ONE 8 (10), E80217 (2013)
  PUBMED    24205392
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 6174)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6174)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2013"; volume: "8"; issue: "10"; pages: "E80217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          161..451
                     /gene="IgapdhI"
     gene            161..451
                     /gene="IgapdhI"
                     /label=IgapdhI
     primer_bind     451..467
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             488..1204
                     /label=EGFP
                     /note="enhanced GFP"
     intron          1244..1756
                     /gene="IgapdhI-II"
     gene            1244..1756
                     /gene="IgapdhI-II"
                     /label=IgapdhI-II
     CDS             1757..2779
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     intron          2783..3300
                     /gene="IgapdhII"
     gene            2783..3300
                     /gene="IgapdhII"
                     /label=IgapdhII
     promoter        complement(3337..3355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3376..3392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3400..3416)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3424..3454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3469..3490)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3778..4366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4540..5397)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5398..5502)
                     /label=AmpR promoter
     rep_origin      complement(5528..5983)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6125..6141
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6148..6166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"