pTEX2 vector (V002748)

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LOCUS       V002748                 5874 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002748
VERSION     V002748
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Meiers M, Laux A, Eichinger D, Sexauer A, Marx P, Bertram R,
            Bruckner R.
  TITLE     A tetracycline inducible integrative expression system for
            Streptococcus pneumoniae
  JOURNAL   FEMS Microbiol. Lett. (2017) In press
   PUBMED   28333198
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Bruckner R.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2016) Microbiology, University of Kaiserslautern,
            Paul-Ehrlich Str. 24, Kaiserslautern, RP D-67663, Germany
REFERENCE   3  (bases 1 to 5874)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5874)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett. (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-NOV-2016) Microbiology, University of Kaiserslautern,
            Paul-Ehrlich Str. 24, Kaiserslautern, RP D-67663, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..70
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    complement(87..105)
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             212..835
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     misc_recomb     910..1894
                     /label="integration site S. pneumoniae"
                     /note="integration site S. pneumoniae"
     misc_feature    2036..2176
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2362..2950)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3124..3981)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3982..4086)
                     /label="AmpR promoter"
     misc_recomb     4191..5252
                     /label="integration site S. pneumoniae"
                     /note="integration site S. pneumoniae"
     CDS             complement(5303..5797)
                     /gene="dfrA13"
                     /label="Dihydrofolate reductase type A13"
                     /note="Dihydrofolate reductase type A13 from Escherichia
                     coli. Accession#: Q59408"