pTET15 vector (V002758)

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LOCUS       V002758                 6151 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002758
VERSION     V002758
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Huber J, Donald RG, Lee SH, Jarantow LW, Salvatore MJ, Meng X,
            Painter R, Onishi RH, Occi J, Dorso K, Young K, Park YW, Skwish S,
            Szymonifka MJ, Waddell TS, Miesel L, Phillips JW, Roemer T.
  TITLE     Chemical genetic identification of peptidoglycan inhibitors
            potentiating carbapenem activity against methicillin-resistant
            Staphylococcus aureus
  JOURNAL   Chem. Biol. 16 (8), 837-848 (2009)
   PUBMED   19716474
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Trawick J, Haselbeck B.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6151)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem.
            Biol."; date: "2009"; volume: "16"; issue: "8"; pages: "837-848"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-MAR-2009) Infectious Diseases, Merck "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    210..231
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        246..276
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    284..300
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..324
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(352..975)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    997..1015
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     regulatory      1046..1107
                     /label="hybrid xylose/tetracycline promoter"
                     /note="hybrid xylose/tetracycline promoter"
                     /regulatory_class="promoter"
     protein_bind    1072..1089
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     terminator      1324..1410
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1502..1529
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(1675..2532)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2533..2637)
                     /label="AmpR promoter"
     rep_origin      2857..3445
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4245..4892)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     rep_origin      5049..5212
                     /note="for replication in Staphylococcus sp."