pTDisp vector (V002759)

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LOCUS       40924_42949        5511 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phage display vector pTDisp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Kolesnikov AV, Kozyr AV, Zakharova MY.
  TITLE     Improved purification of filamentous bacteriophages for phage 
            display using size-exclusion chromatography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Kolesnikov AV, Kozyr AV, Zakharova MY.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and 
            Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 
            16/10, Moscow 117997, Russia
REFERENCE   3  (bases 1 to 5511)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5511)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2004) Laboratory of Biocatalysis, Shemyakin and Ovchinnikov 
            Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, Moscow 
            117997, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(145..1224)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1225..1302)
                     /label=lacI promoter
     protein_bind    1472..1488
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1539..1561
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1683..2231
                     /gene="pIII"
                     /note="m13 minor coat protein fragment; this fragment of
                     the protein is used for N-terminal fusion expression during
                     phage display"
     gene            1683..2231
                     /gene="pIII"
                     /label=pIII
     RBS             2251..2273
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     2281..2346
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     terminator      2566..2652
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2744..2771
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      2869..3324
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3606..3710
                     /label=AmpR promoter
     CDS             3711..4568
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4742..5330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5333..5339
                     /label=ColE1 ori
                     /note="ColE1 ori"