pTCS vector (V002764)

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LOCUS       V002764                 3018 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002764
VERSION     V002764
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ohashi-Kunihiro S, Yohda M, Masaki H, Machida M.
  TITLE     A novel vector for positive selection of inserts harboring an open
            reading frame by translational coupling
  JOURNAL   BioTechniques 43 (6), 751-752 (2007)
   PUBMED   18251250
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Ohashi S, Machida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2007) Contact:Sumiko Ohashi National Institute of
            Advanced Industrial Science and Technology (AIST), Research
            Institute for Cell Engineering; Central 6, 1-1, Higashi, Tsukuba,
            Ibaraki 305-8566, Japan
REFERENCE   3  (bases 1 to 3018)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "BioTechniques"; date: "2007"; volume: "43"; issue: "6"; pages:
            "751-752"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2007) Contact:Sumiko Ohashi National Institute of Advanced
            Industrial Science and Technology (AIST), Research Institute for
            Cell Engineering; Central 6, 1-1, Higashi, Tsukuba, Ibaraki
            305-8566, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(413..667)
                     /gene="imm"
                     /label="Colicin-E3 immunity protein"
                     /note="Colicin-E3 immunity protein from Escherichia coli.
                     Accession#: P02984"
     primer_bind     complement(797..813)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(821..837)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(845..875)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(890..911)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1199..1787)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1961..2818)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2819..2923)
                     /label="AmpR promoter"