pTCH-RS2 vector (V002791)

LOCUS       40924_42774        7672 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pTCH-RS2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Shao M, Blechl A, Thomson JG.
  TITLE     Small Serine Recombination Systems ParA-MRS and CinH-RS2 Perform 
            Precise Excision of Plastid DNA
  JOURNAL   Plant Biotechnol. J. (2017) In press
  PUBMED    28421718
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Shao M, Blechl A, Thomson JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2017) Crop Improvement and Utilization, USDA, 800 
            Buchanan Street, Albany, CA 94710, USA
REFERENCE   3  (bases 1 to 7672)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7672)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JAN-2017) Crop Improvement and Utilization, USDA, 800 Buchanan 
            Street, Albany, CA 94710, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..824
                     /note="plastid sequence targeting insertions in the
                     trnI-trnA intergenic region II; similar to INSD accession 
                     Z00044"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1835..2692)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2693..2797)
                     /label=AmpR promoter
     misc_feature    3021..4438
                     /note="plastid sequence targeting insertions in the
                     trnI-trnA intergenic region I; similar to INSD accession 
                     Z00044"
     regulatory      4470..4586
                     /note="plastid rRNA operon PEP promoter; Prrn"
                     /regulatory_class="promoter"
     regulatory      4591..4607
                     /note="psbA 5' UTR; similar to NC_001879"
                     /regulatory_class="attenuator"
     CDS             4610..5398
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     protein_bind    complement(5445..5478)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_recomb     5517..5622
                     /note="ParA resolution site-MRS; similar to INSD accession 
                     M59825"
     misc_recomb     5634..5752
                     /note="CinH resolution site-RS2; similar to INSD accession 
                     AF213017"
     misc_recomb     5792..5843
                     /note="Bxb1 attP52 recognition site; similar to INSD
                     accession JQ305140"
     misc_recomb     5904..5960
                     /note="phiC31 attP57 recognition site; similar to INSD
                     accession GU56446"
     regulatory      6017..6166
                     /note="rps16; rhct1 mRNA 3' maturation; similar to
                     NC_001879"
                     /regulatory_class="terminator"
     regulatory      6176..6278
                     /note="psbA; similar to NC_001879"
                     /regulatory_class="promoter"
     regulatory      6279..6363
                     /note="psbA 5' UTR; similar to NC_001879"
                     /regulatory_class="attenuator"
     CDS             6378..7046
                     /label=DsRed1
                     /note="wild-type DsRed"
     misc_recomb     7062..7180
                     /note="similar to INSD accession JQ305140; spacer region
                     has been modified to not match the original RS2; engineered
                     to inhibit homologous recombination in chloroplast; CinH 
                     resolution site recognition site - RS2*"
     regulatory      7205..7395
                     /note="psbA 3' UTR; similar to NC_001879"
                     /regulatory_class="terminator"
     misc_feature    7441..7619
                     /note="plastid DNA targeting sequence; similar to inverted 
                     repeat sequence from INSD accession Z00044"