Basic Vector Information
- Vector Name:
- pTCCR-Auto
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12404 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Wu G, Wang Y, Zhu Z.
- Promoter:
- CMV
pTCCR-Auto vector Vector Map
pTCCR-Auto vector Sequence
LOCUS V002799 12404 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V002799
VERSION V002799
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 12404)
AUTHORS Wu G, Wang Y, Zhu Z.
TITLE Direct Submission
JOURNAL Submitted (09-MAR-2004) Fish Genetics, Institute of Hydrobiology,
Chinese Academy of Science, 7# South Road of East Lake, Wuhan, Hubei
430072, People's Republic of China
REFERENCE 2 (bases 1 to 12404)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 12404)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(09-MAR-2004) Fish Genetics, Institute of Hydrobiology, Chinese
Academy of Science, 7# South Road of East Lake, Wuhan, Hubei 430072,
People's Republic of China"
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12404
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(419..452)
/label="lox71"
/note="Left element (LE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
polyA_signal complement(465..586)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(710..1387)
/label="DsRed1"
/note="wild-type DsRed"
promoter complement(1469..1672)
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(1673..1976)
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
terminator complement(2074..2321)
/label="CYC1 terminator"
/note="transcription terminator for CYC1"
CDS complement(2390..2761)
/label="BleoR"
/note="antibiotic-binding protein"
promoter complement(2780..2827)
/label="EM7 promoter"
/note="synthetic bacterial promoter"
regulatory complement(2860..2990)
/label="T7 terminator"
/note="T7 terminator"
/regulatory_class="terminator"
terminator 2880..2927
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(3069..4097)
/label="Cre"
/note="site-specific recombinase"
misc_feature complement(4109..4683)
/note="internal ribosome entry sequence from
encephalomyocarditis virus"
misc_feature 4109..4680
/label="IRES"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
protein_bind 4719..4743
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4744..4762)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory complement(4745..4762)
/label="T7 promoter"
/note="T7 promoter"
/regulatory_class="promoter"
terminator complement(4857..4904)
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(5012..7660)
/note="T7 RNA polymerase from Escherichia phage T7.
Accession#: P00573"
/label="T7 RNA polymerase"
CDS complement(7670..7690)
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
misc_feature complement(7696..8273)
/label="IRES2"
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
protein_bind complement(8312..8336)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8337..8355)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 8710..8731
/label="CAP binding site"
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(8747..9826)
/label="lacI"
/note="lac repressor"
promoter complement(9827..9904)
/label="lacI promoter"
protein_bind complement(10113..10146)
/label="lox66"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
primer_bind complement(10183..10199)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 10207..10223
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10231..10261)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(10276..10297)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(10585..11173)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(11347..12204)
/label="AmpR"
/note="beta-lactamase"
promoter complement(12205..12309)
/label="AmpR promoter"
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