pTBR101-CM vector (V002806)

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LOCUS       V002806                 8365 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002806
VERSION     V002806
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Hirayama Y, Sakanaka M, Fukuma H, Murayama H, Kano Y, Fukiya S,
            Yokota A.
  TITLE     Development of a Double-Crossover Markerless Gene Deletion System in
            Bifidobacterium longum: Functional Analysis of the
            alpha-Galactosidase Gene for Raffinose Assimilation
  JOURNAL   Appl. Environ. Microbiol. 78 (14), 4984-4994 (2012)
   PUBMED   22582061
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Hirayama Y, Sakanaka M, Fukuma H, Murayama H, Kano Y, Fukiya S,
            Yokota A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2011) Contact:Satoru Fukiya Hokkaido University,
            Laboratory of Microbial Physiology, Research Faculty of Agriculture;
            Kita 9, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan
REFERENCE   3  (bases 1 to 8365)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8365)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "14";
            pages: "4984-4994"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-DEC-2011) Contact:Satoru Fukiya Hokkaido University, Laboratory
            of Microbial Physiology, Research Faculty of Agriculture; Kita 9,
            Nishi 9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(109..756)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    1046..1393
                     /label="derived from Escherichia coli plasmid pBR322"
                     /note="derived from Escherichia coli plasmid pBR322"
     misc_feature    1104..1393
                     /gene="tetA"
                     /note="tetA was disrupted by the insertion of
                     Bifidobacterium longum BK25 plasmid pTB4"
     gene            1104..1393
                     /gene="tetA"
                     /label="tetA"
     misc_feature    1394..4351
                     /note="derived from Bifidobacterium longum BK25 plasmid
                     pTB4"
     CDS             complement(1689..2249)
                     /codon_start=1
                     /gene="pTB4_p1"
                     /product="hypothetical protein"
                     /label="pTB4_p1"
                     /protein_id="BAL42613.1"
                     /translation="MTNQLLTPAQCPAHRRRIRDAVPGRVQAGHRAQRLGAGTGVRAPL
                     PLLCGRALPAHGREHPRQRDDAAQGAQGEGRWKMTFQKKEEPLPVRGRRHHPERSSTLM
                     RFPMRSDGYIHGHPHGHPHGHLHGHLHGHLHGHLHGHLHGHPHGDVHQIEGKQARRFMY
                     MNRARSLPRPSAQEQQPECRRPE"
     gene            complement(1689..2249)
                     /gene="pTB4_p1"
                     /label="pTB4_p1"
     CDS             2402..3325
                     /codon_start=1
                     /gene="repA"
                     /product="plasmid replication initiation protein RepA"
                     /label="repA"
                     /protein_id="BAL42614.1"
                     /translation="MSNEYVQGTLELTRAFDGWWLPQRPLCCDDDYSQLVRRSRIDALR
                     CKHIEANPAAQVNMLVVDVDSSEGRLMSLWGHRDMPPNFIAENPANGHCHAGWVLTEPV
                     CRTDMARLKPLKLLHAVTEGLRRSVDGDEGYSGLLMKNPLSDAWDSDLCREDTYDLPDL
                     VAALEAHGDMPPKSWTRTKRAREVGVGRNCTLFDEARTLAYREVRRLPDRTPASSDLLR
                     EYVRRTCHEINASFPDPLPVREVNDTAKSIHKWITTRSRMWRDGAVANAATFVAIQSAR
                     GKKSGEARQNAFEEKFAQYAQEVLGQ"
     gene            2402..3325
                     /gene="repA"
                     /label="repA"
     CDS             3322..3612
                     /codon_start=1
                     /gene="repB"
                     /product="plasmid replication initiation protein RepB"
                     /label="repB"
                     /protein_id="BAL42615.1"
                     /translation="MTIQTIRKKRPLPAKELAEAYGVSVRTIKYWNSQTREDWIDEQAT
                     LRESIRAYHDDDGHSWSQTAEHFNMTQGAVRQRAYRARKEREAEAKAARPE"
     gene            3322..3612
                     /gene="repB"
                     /label="repB"
     CDS             complement(3813..4094)
                     /codon_start=1
                     /gene="pTB4_p2"
                     /product="hypothetical protein"
                     /label="pTB4_p2"
                     /note="Similar to Blon_0217 (Ribbon-helix-helix protein,
                     copG family) [Bifidobacterium longum subsp. infantis ATCC
                     15697]"
                     /protein_id="BAL42616.1"
                     /translation="MSDFSERDRQLMALVGLTEEQVLEDERMAESETVPDDLTGRVYYG
                     LHLAEPDEQMVSVSVRMPKSTLDGLTAMARRYHISRSEYMRRKLAGAI"
     gene            complement(3813..4094)
                     /gene="pTB4_p2"
                     /label="pTB4_p2"
     CDS             complement(4091..4333)
                     /codon_start=1
                     /gene="pTB4_p3"
                     /product="hypothetical protein"
                     /label="pTB4_p3"
                     /protein_id="BAL42617.1"
                     /translation="MERHPELSEKDVVTAFRSVMVDAERESGAWMAIGLDGRGRNVEML
                     YRVVGDLVVIYHAFTPPTKKFRREIDRLRGDGRTL"
     gene            complement(4091..4333)
                     /gene="pTB4_p3"
                     /label="pTB4_p3"
     misc_feature    4352..5252
                     /gene="tetA"
                     /note="tetA was disrupted by the insertion of
                     Bifidobacterium longum BK25 plasmid pTB4"
     gene            4352..5252
                     /gene="tetA"
                     /label="tetA"
     CDS             5891..6079
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    6184..6324
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6510..7098)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7272..8129)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8130..8234)
                     /label="AmpR promoter"