pTBEV-32.11 ic vector (V002807)

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LOCUS       40924_42694       14535 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTBEV-32.11 ic, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14535)
  AUTHORS   de Fabritus L, Nougairede A, Aubry F, Gould EA, de Lamballerie X.
  TITLE     Attenuation of Tick-Borne Encephalitis Virus Using Large-Scale 
            Random Codon Re-encoding
  JOURNAL   PLoS Pathog. 11 (3), E1004738 (2015)
  PUBMED    25734338
REFERENCE   2  (bases 1 to 14535)
  AUTHORS   de Fabritus L, Nougairede A, de Lamballerie X.
  TITLE     Tick-Borne Encephalitis virus TBEV Oshima 32.11 infectious clone
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14535)
  AUTHORS   de Fabritus L, Nougairede A, de Lamballerie X.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2013) UMR_D 190 'Emergence des Pathologies 
            Virales', Aix-Marseille Univ, 27 Bd Jean Moulin, Marseille 13385, 
            France
REFERENCE   4  (bases 1 to 14535)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 14535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2015"; volume: "11"; issue: "3"; pages: "E1004738"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (04-SEP-2013) UMR_D 190 'Emergence des Pathologies Virales', 
            Aix-Marseille Univ, 27 Bd Jean Moulin, Marseille 13385, France"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..131
     CDS             complement(6037..6054)
                     /label=6xHis
                     /note="6xHis affinity tag"
     3'UTR           10377..11100
     ncRNA           11101..11184
                     /product="hepatitis delta ribozyme"
                     /label=ribozyme
                     /ncRNA_class="ribozyme"
     regulatory      11192..11212
                     /regulatory_class="polyA_signal_sequence"
     CDS             11495..11683
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    11788..11928
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(12114..12702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12876..13733)
                     /label=AmpR
                     /note="beta-lactamase"
     enhancer        13939..14317
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14318..14521
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"