Basic Vector Information
- Vector Name:
- pTARG
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7664 bp
- Type:
- P element transformation vector
- Replication origin:
- ori
- Source/Author:
- Egli D, Yepiskoposyan H, Selvaraj A, Balamurugan K, Rajaram R, Simons A, Multhaup G, Mettler S, Vardanyan A, Georgiev O, Schaffner W.
pTARG vector Vector Map
pTARG vector Sequence
LOCUS 40924_42679 7664 bp DNA circular SYN 18-DEC-2018 DEFINITION P element transformation vector pTARG, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7664) AUTHORS Egli D, Yepiskoposyan H, Selvaraj A, Balamurugan K, Rajaram R, Simons A, Multhaup G, Mettler S, Vardanyan A, Georgiev O, Schaffner W. TITLE A family knockout of all four Drosophila metallothioneins reveals a central role in copper homeostasis and detoxification JOURNAL Mol. Cell. Biol. 26 (6), 2286-2296 (2006) PUBMED 16508004 REFERENCE 2 (bases 1 to 7664) AUTHORS Egli D, Georgiev O, Schaffner W. TITLE Direct Submission JOURNAL Submitted (28-OCT-2005) Institute of Molucular Biology, University of Zurich, Winterthurer Street 190, Zurich 8057, Switerland REFERENCE 3 (bases 1 to 7664) TITLE Direct Submission REFERENCE 4 (bases 1 to 7664) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell. Biol."; date: "2006"; volume: "26"; issue: "6"; pages: "2286-2296" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (28-OCT-2005) Institute of Molucular Biology, University of Zurich, Winterthurer Street 190, Zurich 8057, Switerland" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7664 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..8 /label=Polylinker /note="Polylinker" protein_bind 20..67 /label=FRT /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." protein_bind 94..127 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 143..264 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 476..523 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" regulatory 720..945 /label=3xP3-hsp70 promoter /note="3xP3-hsp70 promoter" /regulatory_class="promoter" protein_bind complement(951..984) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." CDS 1178..3238 /label=mini-white /note="Drosophila white gene eye color pigment" misc_feature 3900..3952 /label=flippase recombinase target /note="flippase recombinase target" protein_bind 3905..3952 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." misc_feature complement(4086..4671) /label=P element 5' end rep_origin complement(4906..5494) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5668..6525) /label=AmpR /note="beta-lactamase" promoter complement(6526..6630) /label=AmpR promoter misc_feature complement(7432..7664) /label=P element 3' end /note="P element 3' end"
This page is informational only.