pT7XbG3 vector (V002842)

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LOCUS       40924_42394        3143 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pT7XbG3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Kikawada T, Kikuta S.
  TITLE     Expression vector for synthesis of artificial capped RNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Kikawada T, Kikuta S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2018) Division of Biotechnology, Institute of 
            Agrobiological Sciences, National Institute of Agriculture and Food 
            Research Organization, 1-2 Ohwashi, Tsukuba, Ibaraki 305-0851, Japan
REFERENCE   3  (bases 1 to 3143)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3143)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2018) Division of Biotechnology, Institute of Agrobiological
            Sciences, National Institute of Agriculture and Food Research 
            Organization, 1-2 Ohwashi, Tsukuba, Ibaraki 305-0851, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           659..686
                     /gene="beta-globin"
     misc_feature    687..737
                     /gene="beta-globin"
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           754..879
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     promoter        complement(955..973)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(994..1010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1018..1034)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1042..1072)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1087..1108)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1396..1984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2158..3015)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3016..3120)
                     /label=AmpR promoter