pT7-M-ASKA vector (V002848)

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LOCUS       V002848                 8270 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002848
VERSION     V002848
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Bergeron E, Zivcec M, Chakrabarti AK, Nichol ST, Albarino CG,
            Spiropoulou CF.
  TITLE     Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus
            Reveals a Function for Non-structural Glycoproteins Cleavage by
            Furin
  JOURNAL   PLoS Pathog. 11 (5), E1004879 (2015)
   PUBMED   25933376
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Bergeron E, Chakrabarti AK, Nichol ST, Albarino CG, Spiropoulou CF.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Viral Special Pathogens Branch, Centers for
            Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA
            30333, USA
REFERENCE   3  (bases 1 to 8270)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8270)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog."; date: "2015"; volume: "11"; issue: "5"; pages: "E1004879"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2014) Viral Special Pathogens Branch, Centers for Disease
            Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30333, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..46)
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     promoter        705..809
                     /label="AmpR promoter"
     CDS             810..1667
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      2687..2704
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     promoter        2688..2705
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2706..2798
                     /label="viral UTR"
                     /note="viral UTR"
     CDS             2798..7849
                     /gene="GP"
                     /label="Envelopment polyprotein"
                     /note="Envelopment polyprotein from Crimean-Congo
                     hemorrhagic fever virus (strain Nigeria/IbAr10200/1970).
                     Accession#: Q8JSZ3"
     3'UTR           7853..8071
                     /label="viral UTR"
                     /note="viral UTR"
     ncRNA           8072..8165
                     /product="Hepatitis D virus ribozyme"
                     /label="other"
                     /ncRNA_class="other"
     terminator      8224..8270
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"