pT3C vector (V002856)

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LOCUS       40924_42299       10329 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pT3C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Li XL, Skory CD, Ximenes EA, Jordan DB, Dien BS, Hughes SR, Cotta 
            MA.
  TITLE     Expression of an AT-rich xylanase gene from the anaerobic fungus 
            Orpinomyces sp. strain PC-2 in and secretion of the heterologous 
            enzyme by Hypocrea jecorina
  JOURNAL   Appl. Microbiol. Biotechnol. 74 (6), 1264-1275 (2007)
  PUBMED    17225100
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   Li X-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2006) Fermentation Biotechnology Research Unit, 
            National Center for Agricultural Utilization Research, USDA-ARS, 
            1815 N. University Street, Peoria, IL 61604, USA
REFERENCE   3  (bases 1 to 10329)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2007"; volume: "74"; issue: "6"; 
            pages: "1264-1275"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-NOV-2006) Fermentation Biotechnology Research Unit, National 
            Center for Agricultural Utilization Research, USDA-ARS, 1815 N. 
            University Street, Peoria, IL 61604, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /label=AmpR promoter
     CDS             126..983
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1157..1745
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2069..2099
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2206..4420
                     /label=from Hypocrea jecorina cel7A
                     /note="from Hypocrea jecorina cel7A"
                     /regulatory_class="promoter"
     RBS             3933..3941
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4559..4570)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     regulatory      4702..5410
                     /label=from Hypocrea jecorina cel7A
                     /note="from Hypocrea jecorina cel7A"
                     /regulatory_class="terminator"
     promoter        5420..5780
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             5785..6807
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    6826..9648
                     /label=Hypocrea jecorina cel7A 3' region
                     /note="Hypocrea jecorina cel7A 3' region"
     promoter        complement(9686..9704)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9711..9727)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9869..10324
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"