pT14_T14x3_72bp-TALER21 vector (V002898)

Basic Vector Information

Vector Name:
pT14_T14x3_72bp-TALER21
Antibiotic Resistance:
Ampicillin
Length:
10123 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
Promoter:
lac

pT14_T14x3_72bp-TALER21 vector Vector Map

pT14_T14x3_72bp-TALER2110123 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000oriAmpRAmpR promoterf1 oriM13 fwdT7 promoterHA-LSAattB45X UASTALER14 bs binding siteCMVminiTALER14 bs binding siteTALER14 bs binding siteTALER14 bs binding siteattB1similar to TALER21SV40 NLSFF3FF3FF3FF3attB2beta-globin poly(A) signalM13 revlac operatorlac promoterCAP binding siteHA-RT3 promoterM13 revlac operatorlac promoterCAP binding site

pT14_T14x3_72bp-TALER21 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_42084       10123 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT14_T14x3_72bp-TALER21, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 10123)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10123)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(984..1841)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1842..1946)
                     /label=AmpR promoter
     rep_origin      complement(1972..2427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2569..2585
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2595..2613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2628..3431
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3510..3535
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    4207..4227
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    4275..4369
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    4383..4399
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     misc_feature    4406..4465
                     /label=CMVmini
                     /note="CMVmini"
     protein_bind    4472..4488
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     protein_bind    4489..4505
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     protein_bind    4506..4522
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     protein_bind    4537..4561
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4614..7949
                     /label=similar to TALER21
                     /note="similar to TALER21"
     CDS             7959..7979
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    8025..8045
                     /label=FF3
                     /note="FF3"
     misc_feature    8051..8071
                     /label=FF3
                     /note="FF3"
     misc_feature    8077..8097
                     /label=FF3
                     /note="FF3"
     misc_feature    8103..8123
                     /label=FF3
                     /note="FF3"
     protein_bind    complement(8156..8180)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8335..8390
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8751..8767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8775..8791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8799..8829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8844..8865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    9038..9874
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(9904..9922)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9943..9959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9967..9983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9991..10021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10036..10057)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."

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