Basic Vector Information
- Vector Name:
- pT14_T14x3_72bp-TALER21
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10123 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
- Promoter:
- lac
pT14_T14x3_72bp-TALER21 vector Map
pT14_T14x3_72bp-TALER21 vector Sequence
LOCUS 40924_42084 10123 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pT14_T14x3_72bp-TALER21, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10123)
AUTHORS Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
TITLE Modular construction of mammalian gene circuits using TALE
transcriptional repressors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10123)
AUTHORS Li Y, Jiang Y, Chen H, Liao W.
TITLE Direct Submission
JOURNAL Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE 3 (bases 1 to 10123)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10123)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10123
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(222..810)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(984..1841)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1842..1946)
/label=AmpR promoter
rep_origin complement(1972..2427)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2569..2585
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2595..2613
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2628..3431
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 3510..3535
/label=SA
/note="splice acceptor site"
protein_bind 4207..4227
/label=attB4
/note="core recombination site for the Gateway(R) BP
reaction"
protein_bind 4275..4369
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 4383..4399
/label=TALER14 bs binding site
/bound_moiety="TALER14 bs"
misc_feature 4406..4465
/label=CMVmini
/note="CMVmini"
protein_bind 4472..4488
/label=TALER14 bs binding site
/bound_moiety="TALER14 bs"
protein_bind 4489..4505
/label=TALER14 bs binding site
/bound_moiety="TALER14 bs"
protein_bind 4506..4522
/label=TALER14 bs binding site
/bound_moiety="TALER14 bs"
protein_bind 4537..4561
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 4614..7949
/label=similar to TALER21
/note="similar to TALER21"
CDS 7959..7979
/codon_start=1
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
misc_feature 8025..8045
/label=FF3
/note="FF3"
misc_feature 8051..8071
/label=FF3
/note="FF3"
misc_feature 8077..8097
/label=FF3
/note="FF3"
misc_feature 8103..8123
/label=FF3
/note="FF3"
protein_bind complement(8156..8180)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 8335..8390
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(8751..8767)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 8775..8791
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8799..8829)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8844..8865
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 9038..9874
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(9904..9922)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(9943..9959)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 9967..9983
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9991..10021)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(10036..10057)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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