pT10sOmpArPDI vector (V002906)

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LOCUS       V002906                 4229 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002906
VERSION     V002906
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4229)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4229)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             74..96
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     104..166
                     /label="OmpA signal peptide"
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     CDS             182..1639
                     /gene="P4hb"
                     /label="Protein disulfide-isomerase"
                     /note="Protein disulfide-isomerase from Rattus norvegicus.
                     Accession#: P04785"
     terminator      1691..1738
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     misc_feature    1943..2083
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2269..2857)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3031..3888)
                     /label="AmpR"
                     /note="beta-lactamase"