pSYFP2-sp vector (V002915)

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LOCUS       40924_41979        4850 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSYFP2-sp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Zhang JY, Lin GM, Xing WY, Zhang CC.
  TITLE     Diversity of Growth Patterns Probed in Live Cyanobacterial Cells 
            Using a Fluorescent Analog of a Peptidoglycan Precursor
  JOURNAL   Front Microbiol 9, 791 (2018)
  PUBMED    29740419
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Zhang J-Y., Lin G-M., Xing W-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, 
            Wuhan, Hubei 430072, 086
REFERENCE   3  (bases 1 to 4850)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 9, 791 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 
            430072, 086"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2308..2334
                     /label=domain linker
                     /note="domain linker"
     CDS             2335..3048
                     /label=SYFP2
                     /note="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
     misc_feature    3049..3078
                     /label=domain linker
                     /note="domain linker"
     CDS             3085..3108
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             3145..3168
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      3179..3226
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             3467..4255
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      4316..4334
                     /regulatory_class="terminator"
     primer_bind     complement(4456..4472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"