pSX vector (V002916)

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LOCUS       40924_41969        6268 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSX, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Owen JG, Ackerley DF.
  TITLE     Characterization of pyoverdine and achromobactin in Pseudomonas 
            syringae pv. phaseolicola 1448a
  JOURNAL   BMC Microbiol. 11 (1), 218 (2011)
  PUBMED    21967163
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Ackerley DF, Owen JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2011) School of Biological Sciences, Victoria 
            University, Kelburn Parade, Wellington, NA 6140, New Zealand
REFERENCE   3  (bases 1 to 6268)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6268)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2011"; volume: "11"; issue: "1"; pages: "218"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-SEP-2011) School of Biological Sciences, Victoria University, 
            Kelburn Parade, Wellington, NA 6140, New Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2014..3093)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3094..3171)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        3401..3429
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3433..3457
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3472..3494
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    3498..3527
                     /note="multiple cloning sited region; contains recognition
                     sites for NdeI, BamHI, XbaI, SalI and HindIII"
     terminator      3730..3816
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3908..3935
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(4011..4841)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      4855..5206
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        5256..5284
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5473..6003
                     /label=GmR
                     /note="gentamycin acetyltransferase"