pSV5HIFNG2 vector (V002937)

Download GeneBank File(.gb)

LOCUS       V002937                 7729 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002937
VERSION     V002937
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7729)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    733..867
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1153..1650)
                     /gene="IFNG"
                     /label="Interferon gamma"
                     /note="Interferon gamma from Homo sapiens. Accession#:
                     P01579"
     misc_feature    complement(1747..2684)
                     /label="intron"
                     /note="intron"
     CDS             complement(2691..2876)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        2920..3277
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3291..3691
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88701.1"
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPP"
     polyA_signal    complement(4129..4177)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4222..5256)
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     promoter        complement(5300..5445)
                     /label="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(5902..6490)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6664..7521)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7522..7626)
                     /label="AmpR promoter"