pSV53hFasR vector (V002944)

Basic Vector Information

Vector Name:
pSV53hFasR
Antibiotic Resistance:
Ampicillin
Length:
9864 bp
Type:
Mammalian expression vector
Replication origin:
ori
Source/Author:
De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
Promoter:
SV40

pSV53hFasR vector Map

pSV53hFasR9864 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600SV40 promotersmall t antigensmall t intronSV40 NLSSV40 poly(A) signalTumor necrosis factor receptor superfamily member 65UTRintronAgnoproteinSV40 promotersmall t antigenSV40 NLSbomoriAmpRAmpR promoter

pSV53hFasR vector Sequence

LOCUS       V002944                 9864 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002944
VERSION     V002944
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9864)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3106..4110)
                     /gene="FAS"
                     /label="Tumor necrosis factor receptor superfamily member
                     6"
                     /note="Tumor necrosis factor receptor superfamily member 6
                     from Homo sapiens. Accession#: P25445"
     misc_feature    complement(4111..4307)
                     /label="5UTR"
                     /note="5UTR"
     misc_feature    complement(4371..5308)
                     /label="intron"
                     /note="intron"
     CDS             complement(5315..5500)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        5572..5901
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5915..6436
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             6636..6656
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7711..7851
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8037..8625)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8799..9656)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9657..9761)
                     /label="AmpR promoter"

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