Basic Vector Information
- Vector Name:
- pSV53hFasR
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9864 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- SV40
pSV53hFasR vector Map
pSV53hFasR vector Sequence
LOCUS V002944 9864 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V002944 VERSION V002944 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 9864) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 9864) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 9864) TITLE Direct Submission REFERENCE 4 (bases 1 to 9864) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..9864 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 82..439 /label="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 453..974 /label="small t antigen" /note="SV40 (simian virus 40) small t antigen" intron 979..1044 /label="small t intron" /note="SV40 (simian virus 40) small t antigen intron" CDS 1174..1194 /label="SV40 NLS" /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 2948..3082 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" CDS complement(3106..4110) /gene="FAS" /label="Tumor necrosis factor receptor superfamily member 6" /note="Tumor necrosis factor receptor superfamily member 6 from Homo sapiens. Accession#: P25445" misc_feature complement(4111..4307) /label="5UTR" /note="5UTR" misc_feature complement(4371..5308) /label="intron" /note="intron" CDS complement(5315..5500) /note="Agnoprotein from Simian virus 40. Accession#: P03084" /label="Agnoprotein" promoter 5572..5901 /label="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 5915..6436 /label="small t antigen" /note="SV40 (simian virus 40) small t antigen" CDS 6636..6656 /label="SV40 NLS" /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" misc_feature 7711..7851 /label="bom" /note="basis of mobility region from pBR322" rep_origin complement(8037..8625) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8799..9656) /label="AmpR" /note="beta-lactamase" promoter complement(9657..9761) /label="AmpR promoter"
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