pSV51 vector (V002960)

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LOCUS       V002960                 9192 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002960
VERSION     V002960
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9192)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9192)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..439
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             453..974
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          979..1044
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1174..1194
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2948..3082
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      complement(3218..3299)
                     /label="fd terminator"
                     /note="fd terminator"
                     /regulatory_class="terminator"
     terminator      3248..3296
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(3538..3586)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     misc_feature    complement(3699..4636)
                     /label="major intron of 16S mRNA of SV40 VP1"
                     /note="major intron of 16S mRNA of SV40 VP1"
     CDS             complement(4643..4828)
                     /note="Agnoprotein from Simian virus 40. Accession#:
                     P03084"
                     /label="Agnoprotein"
     promoter        4900..5229
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5243..5764
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     intron          5768..5834
                     /note="intron of SV40 small t-antigen"
     CDS             5964..5984
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    7039..7179
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7365..7953)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8127..8984)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8985..9089)
                     /label="AmpR promoter"