Basic Vector Information
- Vector Name:
- pSV25S-HTNFR75
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5598 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- SV40
pSV25S-HTNFR75 vector Map
pSV25S-HTNFR75 vector Sequence
LOCUS V002968 5598 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V002968 VERSION V002968 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 5598) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5598) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5598) TITLE Direct Submission REFERENCE 4 (bases 1 to 5598) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5598 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(750..884) /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" CDS complement(1309..1329) /label="SV40 NLS" /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" intron complement(1459..1524) /label="small t intron" /note="SV40 (simian virus 40) small t antigen intron" CDS complement(1526..1600) /codon_start=1 /note="unnamed protein product; part of SV40 small t-antigen" /protein_id="SJL88757.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" CDS complement(1623..3005) /gene="TNFRSF1B" /label="Tumor necrosis factor receptor superfamily member 1B" /note="Tumor necrosis factor receptor superfamily member 1B from Homo sapiens. Accession#: P20333" promoter complement(3027..3356) /label="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin complement(3771..4359) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4533..5390) /label="AmpR" /note="beta-lactamase" promoter complement(5391..5495) /label="AmpR promoter"
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