pSV2-gpt vector (V002994)

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LOCUS       V002994                 5297 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002994
VERSION     V002994
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5297)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL88688.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1608..2004)
                     /label="3' UTR of XGPRT"
                     /note="3' UTR of XGPRT"
     CDS             complement(2008..2463)
                     /gene="gpt"
                     /label="Xanthine-guanine phosphoribosyltransferase"
                     /note="Xanthine-guanine phosphoribosyltransferase from
                     Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
                     Accession#: B7N8G9"
     misc_feature    complement(2464..2654)
                     /label="5' UTR of XGPRT"
                     /note="5' UTR of XGPRT"
     promoter        complement(2664..2993)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    3144..3284
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3470..4058)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4232..5089)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5090..5194)
                     /label="AmpR promoter"