pSV-ssTMbgyg vector (V002996)

LOCUS       40924_41549        9657 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Eukaryotic vector pSV-ssTMbgyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Shirasawa S, Yoshimi M, Kamochi H, Muneta T, Kimura JH, Hook M, 
            Shinomura T.
  TITLE     Gene trap screening for cell surface and extracellular matrix 
            molecules produced by chondrocytes
  JOURNAL   J. Biochem. 137 (1), 79-85 (2005)
  PUBMED    15713886
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   Shinomura T, Shirasawa S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental
            University, Tissue Regeneration, Dept. of Hard Tissue Engineering; 
            1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan 
            (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, 
            Fax:81-3-5803-0187)
REFERENCE   3  (bases 1 to 9657)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9657)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2005"; volume: "137"; issue: "1"; pages: "79-85"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental 
            University, Tissue Regeneration, Dept. of Hard Tissue Engineering"; 
            volume: " 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan 
            (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, Fax"; pages: 
            "81-3-5803-0187"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..375
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          401..973
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1106..1150
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             1151..1174
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    1231..1893
                     /gene="TM(CD4)"
                     /product="transmembrane domain of rat CD4"
     gene            1231..1893
                     /gene="TM(CD4)"
                     /label=TM(CD4)
     CDS             1901..4942
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             4958..5992
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    6037..6085
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      6276..6495
                     /regulatory_class="terminator"
     promoter        complement(6526..6544)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6562..6578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6586..6602)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6610..6640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6655..6676)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6964..7552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7726..8583)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8584..8688)
                     /label=AmpR promoter
     rep_origin      9022..9477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9618..9634
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9641..9657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"