pSV-Sport-Raf-1E vector (V002998)

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LOCUS       V002998                 5246 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002998
VERSION     V002998
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..339
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        345..363
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             395..2338
                     /gene="RAF1"
                     /label="RAF proto-oncogene serine/threonine-protein kinase"
                     /note="RAF proto-oncogene serine/threonine-protein kinase
                     from Pongo abelii. Accession#: Q5R5M7"
     misc_feature    2348..2386
                     /label="E-tag"
                     /note="E-tag"
     promoter        complement(2564..2582)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2671..2710
                     /label="intron of SV40 small t-antigen"
                     /note="intron of SV40 small t-antigen"
     polyA_signal    2969..3103
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3340..3918)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4092..4949)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4950..5054)
                     /label="AmpR promoter"
     promoter        5166..5197
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    5205..5221
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."