pSUNG vector (V003013)

LOCUS       V003013                 8700 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003013
VERSION     V003013
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
   PUBMED   21366919
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8700)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res
            Notes"; date: "2011"; volume: "4"; issue: "1"; pages: "44"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Crop Improvement and Utilization Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    complement(137..184)
                     /label="FRT (mutant)"
                     /note="FRT site with a mutation in the core sequence (Azam
                     et al., 1997)"
     terminator      complement(209..460)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(493..1329)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     transit_peptide complement(1330..1500)
                     /gene="Sul1"
                     /note="Pea rbcS ribulose-bisphosphate carboxylase small
                     subunit transit peptide for plastid targeting"
     promoter        complement(1554..1733)
                     /label="NOS promoter"
                     /note="nopaline synthase promoter"
     intron          1931..2120
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     CDS             2115..3938
                     /label="GUSPlus(TM)"
                     /note="beta-glucuronidase"
     CDS             3945..3962
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3994..4246
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4268..4292
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4473..4497
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4711..5299
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5542..6687)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(6989..7780)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7992..8623)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"