pSUC vector (V003019)

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LOCUS       40924_41405       11715 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Obermaier S, Fuertges L.
  TITLE     Biosynthesis of dimeric naphthopyrones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Obermaier S, Fuertges L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2017) Pharmaceutical Chemistry, University 
            Freiburg, Fahnenbergplatz, Freiburg 79085, Germany
REFERENCE   3  (bases 1 to 11715)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2017) Pharmaceutical Chemistry, University Freiburg, 
            Fahnenbergplatz, Freiburg 79085, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      4..292
                     /regulatory_class="terminator"
     CDS             715..1737
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     protein_bind    complement(1812..1845)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     primer_bind     complement(2315..2331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2601..3189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3316..4173)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4174..4265)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4558..4591
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(4905..10161)
                     /direction=LEFT
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans
                     (Aleksenko and Clutterbuck, 1996)"
     regulatory      10437..11702
                     /regulatory_class="promoter"