pSU22 vector (V003022)

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LOCUS       40924_41390        4398 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSU22 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Nagano Y, Takao S, Kudo T, Iizasa E, Anai T.
  TITLE     Yeast-based recombineering of DNA fragments into plant 
            transformation vectors by one-step transformation
  JOURNAL   Plant Cell Rep. 26 (12), 2111-2117 (2007)
  PUBMED    17680244
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Nagano Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2007) Yukio Nagano, Saga University, Analytical 
            Research Center for Experimental Sciences; Honjo-machi 1, Saga 
            840-8502, Japan (E-mail:nagano@cc.saga-u.ac.jp, Tel:81-952-28-8898, 
            Fax:81-952-28-8896)
REFERENCE   3  (bases 1 to 4398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2007"; volume: "26"; issue: "12"; pages: "2111-2117"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JUL-2007) Yukio Nagano, Saga University, Analytical Research 
            Center for Experimental Sciences"; volume: " Honjo-machi 1, Saga 
            840-8502, Japan (E-mail:nagano@cc.saga-u.ac.jp, Tel:81-952-28-8898, 
            Fax"; pages: "81-952-28-8896"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..893
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1042..1922
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2521..2741
                     /label=URA3 promoter
     CDS             2742..3542
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      3701..4289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"