pSU0 vector (V003023)

LOCUS       40924_41385        4398 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSU0 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Iizasa E, Nagano Y.
  TITLE     Highly efficient yeast-based in vivo DNA cloning of multiple DNA 
            fragments and the simultaneous construction of yeast/ Escherichia 
            coli shuttle vectors
  JOURNAL   BioTechniques 40 (1), 79-83 (2006)
  PUBMED    16454044
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Iizasa E, Nagano Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2005) Contact:Ei'ichi Iizasa Division of Molecular
            and Cellular Immunoscience, Department of Biomolecular Sciences 
            Faculty of Medicine, Saga University; 1-1, 5 chome, Nabeshima, Saga 
            City, Saga 849-8501, Japan
REFERENCE   3  (bases 1 to 4398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2006"; volume: "40"; issue: "1"; pages: 
            "79-83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2005) Contact:Ei'ichi Iizasa Division of Molecular and 
            Cellular Immunoscience, Department of Biomolecular Sciences Faculty 
            of Medicine, Saga University; 1-1, 5 chome, Nabeshima, Saga City, 
            Saga 849-8501, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..893
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1042..1922
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2521..2741
                     /label=URA3 promoter
     CDS             2742..3542
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      3701..4289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"