pSTNSK-Tp vector (V003025)

Download GeneBank File(.gb)

LOCUS       V003025                10870 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003025
VERSION     V003025
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
   PUBMED   22706059
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2012) Microbiology and Biothechnology,
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval,
            Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 10870)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10870)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17";
            pages: "6001-6008"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-JAN-2012) Microbiology and Biothechnology, INRS-Institut
            Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7,
            Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(281..1807)
                     /codon_start=1
                     /gene="tnsD"
                     /product="Tn7 transposase subunit"
                     /label="tnsD"
                     /protein_id="AFM78104.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
                     LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
                     HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
                     AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
                     QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
                     FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
                     LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
                     VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
                     QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            complement(281..1807)
                     /gene="tnsD"
                     /label="tnsD"
     CDS             complement(1813..3477)
                     /gene="tnsC"
                     /label="Transposon Tn7 transposition protein TnsC"
                     /note="Transposon Tn7 transposition protein TnsC from
                     Escherichia coli. Accession#: P05846"
     CDS             complement(3477..5582)
                     /gene="tnsB"
                     /label="Transposon Tn7 transposition protein TnsB"
                     /note="Transposon Tn7 transposition protein TnsB from
                     Escherichia coli. Accession#: P13989"
     mobile_element  6300..6524
                     /label="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     protein_bind    6735..6782
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(6847..7080)
                     /label="TpR"
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(7407..7435)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     misc_recomb     7487..7534
                     /note="FRT; Flp recombinase target site"
     protein_bind    7487..7534
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(7596..7614)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7650..7683)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             7851..8642
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      9511..9733
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9781..10728
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"