pSTH1-GFP vector (V003028)

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LOCUS       40924_41355        7784 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSTH1-GFP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Yamashita M, Hojo M.
  TITLE     Transgenic zebrafish overexpressing HSP70
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Yamashita M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2002) Michiaki Yamashita, National Research 
            Institute of Fisheries Science; 2-12-4 Fukuura, Kanazawa-ku, 
            Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, 
            Tel:81-45-788-7665, Fax:81-45-788-5001)
REFERENCE   3  (bases 1 to 7784)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7784)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2002) Michiaki Yamashita, National Research Institute of 
            Fisheries Science"; volume: " 2-12-4 Fukuura, Kanazawa-ku, Yokohama,
            Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, 
            Tel:81-45-788-7665, Fax"; pages: "81-45-788-5001"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    390..524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      649..1104
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             1392..2249
                     /label=AmpR
                     /note="beta-lactamase"
     protein_bind    2354..2387
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      2465..3053
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3516..3547
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    3555..3571
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      3910..4243
                     /note="rainbow trout heat shock cognate protein HSC70
                     promoter"
                     /regulatory_class="promoter"
     CDS             4402..4446
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     regulatory      6465..6470
                     /regulatory_class="polyA_signal_sequence"
     enhancer        6511..6814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6815..7018
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             7063..7779
                     /label=EGFP
                     /note="enhanced GFP"