pSTE11-URA3 vector (V003029)

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LOCUS       V003029                 6903 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003029
VERSION     V003029
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
   PUBMED   21249169
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 6903)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio";
            date: "2011"; volume: "2"; issue: "1"; pages: "e00230-10"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount Av.,
            Montreal, QC H4P2R2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            415..2889
                     /gene="STE11"
                     /label="STE11"
     CDS             415..2886
                     /codon_start=1
                     /gene="STE11"
                     /product="STE11"
                     /label="STE11"
                     /note="ORF19.844; Candida albicans MAPKKK"
                     /protein_id="ADW40670.1"
                     /translation="MTEINDSTENITRIDRNDAKELKHWLAKANCQHHYSKFIENGITM
                     DLLPELDTGSLKELGISKLGDRLRLEIAISNLKAENLKELISVDEIYQLLNLNPQPSGS
                     LTNLNNNNNSLLDVSNSNEGTLTQLSQNNSSNNLPISSMNSSTNNINGNTINSSSGNVV
                     GNSASSKDSSKTITFILQDGSTKRVNATGCFNAQAIKRRVLKKLGFKPDEIQFDTYVHS
                     VPHGVENVLYGKTEPTITLLYDVELVTICYSPERLEKYRIMLVPKNEPPTPAAIETSKK
                     IMSKYKKQVSGSKAELQEEVNQLHTKDVQAHRSTLRNFFGQRPPSELISSNLAEYFPDT
                     RQKDLEQTVRNSVRHSVRLSRRFNLPAGAFSSSVYSLNKRQSILSNSTGFPSNRTMSIS
                     SGEQQSGVFNTSGGGSGGLRRAQRTIGDVMVNSITAIDEVVNSNDTFSVFNKPSTSFSN
                     QPVQPLNNHDSRSIISSKSLSGGTALHRISISPNYNDNPAHDRHSTIELLNPSDSEGED
                     GEEDGEFGISDYAGLSSSSGENGPPENWLKGARIGSGSFGTVYLGMNPFTGELMAVKQI
                     PLVNENGNNSDTENIQNSMQEQQREMMLLKELNHENIVRYFGSTTDENFLNIFLEYVPG
                     GSVQSMLNSYGPFEEPLIRNFVRQVLIGLSYLHGEDIIHRDIKGANILIDIKGTVKIGD
                     FGISKKVSAIDEEDEDFKKTGKRASLQGSVFWMAPEVVKQTTYTKKADIWSVGCLIVEM
                     FTGRHPFPELSQMQALFKIGNHITPQIPEWCTNEAKEFLKKTFEINFEMRPDAIELLAE
                     QFLNPLIMSKQ"
     CDS             3527..4336
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        complement(4541..4559)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4817..5405)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5579..6436)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6437..6541)
                     /label="AmpR promoter"
     promoter        6887..6903
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"