Basic Vector Information
- Vector Name:
- pSTA
- Antibiotic Resistance:
- Kanamycin
- Length:
- 11849 bp
- Type:
- Cloning vector
- Replication origin:
- RSF ori
- Source/Author:
- Baka E, Varga S, Hobel C, Knispel RW, Fekete C, Ivanics M, Kriszt B, Nagy I, Kukolya J.
pSTA vector Vector Map
pSTA vector Sequence
LOCUS 40924_41320 11849 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pSTA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11849)
AUTHORS Baka E, Varga S, Hobel C, Knispel RW, Fekete C, Ivanics M, Kriszt B,
Nagy I, Kukolya J.
TITLE The first transformation method for the thermo-acidophilic archaeon
Thermoplasma acidophilum
JOURNAL J. Microbiol. Methods 95 (2), 145-148 (2013)
PUBMED 23978635
REFERENCE 2 (bases 1 to 11849)
AUTHORS Baka E, Kukolya J, Nagy I, Varga S, Knispel R.
TITLE Direct Submission
JOURNAL Submitted (27-FEB-2013) Department of Microbiology, Central
Environmental and Food Science Research Institute, Herman Otto Road
15, Budapest 1022, Hungary
REFERENCE 3 (bases 1 to 11849)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11849)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J.
Microbiol. Methods"; date: "2013"; volume: "95"; issue: "2"; pages:
"145-148"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-FEB-2013) Department of Microbiology, Central Environmental and
Food Science Research Institute, Herman Otto Road 15, Budapest 1022,
Hungary"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: Ape Plasmid Editor v. 2.0
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..11849
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..6100
/label=pTA1 plasmid NcoI fragment
/note="pTA1 plasmid NcoI fragment"
misc_feature 1..163
/note="derived from pTA1 plasmid ORF13 from T. acidophilum
of INSD accession AB190359"
CDS complement(122..457)
/codon_start=1
/product="hypothetical membrane protein"
/label=hypothetical membrane protein
/note="derived from pTA1 plasmid ORF14 from T. acidophilum
of INSD accession AB190359"
/protein_id="AGL45415.1"
/translation="MCISMICLCRPVLVSCTCCFPGVCVRILGISLAPCLRSYLGRPLQ
YPFSYLSYYYVLVVKNIGFVVCRFWRPALVADPALRFRLLPFFITLDNVRSAPLFVRGH
IFFGCLI"
CDS 500..808
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="derived from pTA1 plasmid ORF15 from T. acidophilum
of INSD accession AB190359"
/protein_id="AGL45416.1"
/translation="MNSETYILIFIDNLPRKIPTWIAESKNIKEFEHFSLKKGTEKAML
ISNQRLEKWIPVSKTKRIKKENETALEWAIKELNNPEHRNEATAKIRKLINSEEGEQ"
CDS 805..1044
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="derived from pTA1 plasmid ORF16 from T. acidophilum
of INSD accession AB190359"
/protein_id="AGL45417.1"
/translation="MTDLKQLLKDHHPEIKEMPSHKEAIEILLQDLENIYKRMRILYYC
RNCKKIINPGEEYLHFQHILEDAIILQEPPPKRN"
CDS 1004..1381
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="derived from pTA1 plasmid ORF17 from T. acidophilum
of INSD accession AB190359"
/protein_id="AGL45418.1"
/translation="MPLYYKNPHQKEIDGPLIFSPTIITKSNKKNTFNKSMPYQGMKQT
INPEEILHLARIGEIGTIDSYKGQPLIVNPLNDRPISIQYDPHTDSFFATIGSRNIYLT
DKQASRLIRDLTKYLDQITKI"
CDS 1702..2115
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="derived from pTA1 plasmid ORF18 from T. acidophilum
of INSD accession AB190359"
/protein_id="AGL45419.1"
/translation="MPVINANISDEAYTILKEITEQTDGNKSKALTKIILAFKECMENH
QQTASEEHEEDQGSTEDIEQIKKFLQDQTEEAPERYQITKGELYQKFLEYCKKSSHPEI
PKPTFYSIIKRNIKIQDNDNARPRVYKGIKWKE"
CDS 2553..3638
/codon_start=1
/gene="Cdc6"
/product="cell division control protein 6-like protein"
/label=Cdc6
/note="derived from pTA1 plasmid ORF1 from T. acidophilum
of INSD accession AB190359; putative replication initiator"
/protein_id="AGL45420.1"
/translation="MISDKNNLDELTRKTIETLKDNGNNLKDPSVFDLNYQPKKIFIRK
EINAILEQLGQYALWKIPKHIALIGPKGAGKTITIKTIAESLQRTQGLENIYINARETQ
TSYKIYQEITKHYTKGPDLSEMRAKALKRLTDHTLLIIDEVDFLKDQDILYTVTRETKT
TLILLTQKLSWIKNLKDESVASSLQPQLITFNQYTPQELSEILTMRAEEGLYQFDPGSI
NLISALVARNYRGDTRIAIKTLERIGYRNQWNEEEIYKALEEAYNELEGTILRGLSPRD
LEILLIISKTPESNKAYLIAKQSTYARNISRPTFLQALNHLQNLDLIMLTKKRVGKSNT
QEAEILLNNKDLIEKIASEKL"
gene 2553..3638
/gene="Cdc6"
/label=Cdc6
CDS complement(4196..5080)
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="derived from pTA1 plasmid ORF2 from T. acidophilum
of INSD accession AB190359"
/protein_id="AGL45421.1"
/translation="MRSGPCGVLPRIFRISMRAWLIKFSSIFSFSSIHDGDLRRFAMIN
DFLAWAVANLKPYQFIDVRNAYSERLDVPIFTLDYAGFSAYMDAVVGMNWDVRYEDEVM
AVLKNGPASVSDIVDRITSKYPVSRKEASDKVWLVLGRFRDNDMGWADCQGHVLKLRHI
DMDLKHDEGGLWFLSGSNMSVLHDWMEGQVVRVLRDYHVEFAVPPEYTRRRDADIVLHG
FKIEIETGLKKDSSALVDRISSYDGPVVVVVPNQAVKSRYARILSGFQNVNVLTLLEFI
YDLKREYIKSVFK"
CDS 5001..5603
/codon_start=1
/product="hypothetical protein"
/label=hypothetical protein
/note="derived from pTA1 plasmid ORF3 from T. acidophilum
of INSD accession AB190359"
/protein_id="AGL45422.1"
/translation="MLENLISQALIDIRKILGSTPQGPERTDLTHHIVNHCGTHTGQPV
DLIDGQDNRTAPRVYHPPDLTEEKLGIEGFCTLIKAGEIEDVVIIDAKGEIDHRIGRYK
TQVSLYNGPGLTLDPGSIRPAGPHDIQKIIIGRRNHDRRMQERRDLRSGYTLDVNGKGN
IPRINEGITIKKEGIRDPISNPNNTKRKMQIRIIGLK"
CDS 6112..6129
/label=6xHis
/note="6xHis affinity tag"
promoter 6243..6261
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 6262..6286
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 6395..6439
/label=S-Tag
/note="affinity and epitope tag derived from pancreatic
ribonuclease A"
terminator 6491..6538
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(6771..7583)
/label=KanR
/note="aminoglycoside phosphotransferase"
promoter complement(7584..7675)
/label=AmpR promoter
rep_origin complement(7691..8440)
/direction=LEFT
/label=RSF ori
/note="Plasmids containing the RSF 1030 origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
misc_feature 8446..8852
/label=promoter Ta1137/1138
/note="promoter Ta1137/1138"
CDS 8856..10781
/codon_start=1
/gene="gyrB"
/product="gyrase B"
/label=gyrB
/note="Ta10555"
/protein_id="AGL45423.1"
/translation="MTEDNYDSSQIQILEGLKAVRKVPGMYIGSTDTRGLHHLVYEVVD
NSVDESVAGYCSRIYVVMGSDGSITVEDDGRGIPVDIHPKYNRPGLEIVLTELHSGAKF
DKKVYKLTGGLHGVGLHVVNALSKKLIAVVKRDGKIYYDIFEQGIPVSGLKTASDVSEI
EKLGIKIQFPEHGAIIKFYPDPDIFETTEFSYETILARLTDLSYLNPQLTITFLDEASG
RKDILHHEGGLIELVRHLSEGKEALMEPLYLKEEVDSHMVEFSLLYTTDVQETLMSFVN
NISTPEGGTHVAGFHQGLSRAIQDYARSNNKIKGVEDITGDDVKEGVIAVLHVKMQNPQ
FEGQTKSKLGNSSVRGIVQSVTAKFMKTFMETNPHVADVIIGRVLSAAAAREASRKAKE
LVRRKSALEGGGLPGKLADCSSNDPSNSELYIVEGDSAGGSAKQARNRQYQAVLPLRGK
ILNVEKASDMKVVENEIIHDLVVAIGTGVKEDLNPKKLRYGKIIIMTDADVDGAHIRTL
LLTFFFRYARSLIENGNVFFAEPPLYRIQKGQNVRYVYSDEEKEQVSREFGPNAIIQRF
KGLGEMNPEQLWETTMNPKTRKLVQVTIEDAEEAERIFTILMGEKVEPRRKFIEENAVY
AENIDL"
gene 8856..10781
/gene="gyrB"
/label=gyrB
misc_feature 8856..9022
/gene="gyrB"
/note="Ta1055; derived from INSD accession AL445066"
misc_feature 9023..9993
/gene="gyrB"
/label=derived from INSD accession AB206999
/note="derived from INSD accession AB206999"
misc_feature 9994..10781
/gene="gyrB"
/note="Ta1055; derived from INSD accession AL445066"
promoter complement(11639..11716)
/label=lacI promoter
promoter 11762..11780
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 11781..11805
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 11820..11842
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
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