pST98-AS vector (V003035)

MSDS Download GenBank File(.gb)

Basic Vector Information

pSC101 ori is a low-copy replication origin that requires the Rep101 protein, which is a temperature-sensitive protein. When bacteria containing a plasmid with this ori is cultured at 37℃, the plasmid will be lost.

Vector Name:: pST98-AS

Antibiotic Resistance:: Ampicillin

Length:: 4598 bp

Type:: Cloning vector

Replication origin:: pSC101 ori

Source/Author:: Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.

Copy Number:: Low Copy

Promoter:: tetR/tetAs

Growth Temperature:: 30℃

pST98-AS vector Map

Copy Sequence View Map

pST98-AS vector Sequence

LOCUS       V003035                 4598 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003035
VERSION     V003035
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Markerless gene replacement in Escherichia coli stimulated by a
            double-strand break in the chromosome
  JOURNAL   Nucleic Acids Res. 27 (22), 4409-4415 (1999)
   PUBMED   10536150
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center
            Hung. Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary
REFERENCE   3  (bases 1 to 4598)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4598)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1999"; volume: "27"; issue: "22"; pages:
            "4409-4415"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center Hung.
            Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..697)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        713..768
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             782..1474
                     /gene="SCEI"
                     /label="Intron-encoded endonuclease I-SceI"
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P03882"
     promoter        1525..1543
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1551..1635
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     misc_feature    1588
                     /label="I-SceI cleavage site"
                     /note="I-SceI cleavage site"
     CDS             complement(1767..2714)
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     rep_origin      complement(2762..2984)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(3609..4466)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4467..4571)
                     /label="AmpR promoter"

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